DNA

Part:BBa_K817016:Design

Designed by: Shan Chi Hsieh   Group: iGEM12_NTU-Taida   (2012-09-18)

parS site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It had better be used in low copy plasmid.

This part is cloned by primer ligation, because of it's short sequence.

IR2-F:AATTCGCGGCCGCTTCTAGAGGGTTCCACGTGGAACATA
IR2-R:CTAGTATGTTCCACGTGGAACCCTCTAGAAGCGGCCGCG

Mix these two primers, it will anneal and generate speI, EcoRI cutting sites.

IR2-F:5' AATTCGCGGCCGCTTCTAGAGGGTTCCACGTGGAACATA 3'
IR2-R:3' GCGCCGGCGAAGATCTCCCAAGGTGCACCTTGTATGATC 5'

Then dilute these primers to 1 uM(each), directly ligate 1ul with vector that have been cut by speI and EcoRI.

Source

pseudomonas putida KT2440 chromosome

References